Cryo EM Course

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Cryo EM Courses

LBMS hosts cryo-EM courses with leaders in the field to educate users in the cutting edge science of cryo-EM. Every talk has a different focus and perspective on sample prep and/or imaging. Whether you are just beginning in cryo-EM, or an expert, through each course you will gain invaluable information directly from the leaders in the field.

Course Name
Course Date
Course Location
Cryo-EM Zoom Course
6/15-6/18, 2021
Virtual
TBD
TBD
TBD

Cryo EM Virtual Course(June 15th - June 18th, 2021)

Course Homepage

Please visit the following link to access the course homepage: Course Homepage

Motivation

A key goal of LBMS is to organize, nurture and expand the cryo-EM community for Department of Energy (DOE)-funded researchers. This powerful and versatile technique will help to advance our understanding of the biological complexity of plant and microbial metabolism and their interfaces across scales spanning molecules to ecosystems, and to develop technologies to identify DOE mission-relevant metabolic capabilities and engineering possibilities in bacteria, fungi, archaea, viruses, plants and mixed communities.

This cryo-EM course is a major element in our DOE Outreach activities. The aim is to attract researchers from this community to take advantage of LBMS’ cryo-EM and help them in all phases including project initiation, sample preparation, data collection, and data processing and interpretation.>/span>

Course Topics

•Introduction to LBMS

•Introduction to electron microscopy and structural biology

•Introduction to electron microscope and image formation process

•Introduction to negative staining and cryo-electron microscopy •Single-particle sample preparation tutorial and demonstration (negative staining and cryogenic vitrification)

•Single particle reconstruction: theory, application and available software

•MicroED: theory, application and available software

•Cryo-electron tomography

•EPU single particle data collection tutorial and demonstration

•Model building, refinement, and validation.

•Cryo-FIB sample preparation tutorial and demonstration

•SerialEM electron tomography data collection tutorial and demonstration

•Single-particle data analysis workflow tutorial and demonstration

•Tomographic data analysis workflow including subtomography average tutorial and demonstration.

Invited Speakers

•Muyan Chen (BCM)

•Oliver Clarke (Columbia)

•Mark Ebrahim (Rockefeller)

•Tamir Gonen (UCLA)

•Jianfeng Lin (Yale)

•Raphael Park (Yale)

•Fred Sigworth (Yale)

Session Video Recordings

Introduction to LBMS Speaker: Liguo Wang

Introduction to electron microscopy and structural biology Speaker: Fred Sigworth

Introduction to electron microscope and image formation process Speaker: Mark Ebrahim

Introduction to negative staining and cryo-electron microscopy Speaker: Liguo Wang

Single-particle sample preparation tutorial and demonstration (negative staining and cryogenic vitrification) Speaker: Guobin Hu

Single particle reconstruction: theory, application and available software Speaker: Fred Sigworth

Model building, refinement, and validation Speaker: Oliver Clarke

Single-particle data analysis workflow tutorial and demonstration Speaker: Dongyan Tan

EPU single particle data collection tutorial and demonstration Speaker: Guobin Hu

Introduction to Cryo-electron tomography Speaker: Jun Liu

Cryo-ET sample preparation tutorial and demonstration Speaker: Jianfeng Lin

Cryo-ET data collection and reconstruction tutorial and demonstration Speaker: Jun Liu

Tomographic data segmentation tutorial and demonstration Speaker: Raphael Park

Subtomography averaging tutorial and demonstration in EMAN2 Speaker: Muyan Chen

Discussion Speaker: Qun Liu and Yong Xiong


Committee Information

•Liguo Wang: LBMS at Brookhaven National Laboratory

•Guobin Hu: LBMS at Brookhaven National Laboratory

•Qun Liu: Biology department at Brookhaven National Laboratory

•Yong Xiong: Professor, Dept. of Molecular Biophysics and Biochemistry, Yale University

•Dongyan Tan: Assistant Professor, Pharmacological Sciences, Stony Brook University

•Jun Liu: Associate Professor, Department of Microbial Pathogenesis, Yale University

•Sean McSweeney NSLS-II at Brookhaven National Laboratory