Cryo EM Course

LBMS|Laboratory for BioMolecular Structure

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Cryo EM Courses

LBMS hosts cryo-EM courses with leaders in the field to educate users in the cutting edge science of cryo-EM. Every talk has a different focus and perspective on sample prep and/or imaging. Whether you are just beginning in cryo-EM, or an expert, through each course you will gain invaluable information directly from the leaders in the field.

Course Name	Course Date	Course Location
Cryo-EM Zoom Course	6/15-6/18, 2021	Virtual
TBD	TBD	TBD

Cryo EM Virtual Course(June 15th - June 18th, 2021)

Course Homepage

Please visit the following link to access the course homepage: Course Homepage

Motivation

A key goal of LBMS is to organize, nurture and expand the cryo-EM community for Department of Energy (DOE)-funded researchers. This powerful and versatile technique will help to advance our understanding of the biological complexity of plant and microbial metabolism and their interfaces across scales spanning molecules to ecosystems, and to develop technologies to identify DOE mission-relevant metabolic capabilities and engineering possibilities in bacteria, fungi, archaea, viruses, plants and mixed communities.

This cryo-EM course is a major element in our DOE Outreach activities. The aim is to attract researchers from this community to take advantage of LBMS’ cryo-EM and help them in all phases including project initiation, sample preparation, data collection, and data processing and interpretation.>/span>

Course Topics

•Introduction to LBMS

•Introduction to electron microscopy and structural biology

•Introduction to electron microscope and image formation process

•Introduction to negative staining and cryo-electron microscopy •Single-particle sample preparation tutorial and demonstration (negative staining and cryogenic vitrification)

•Single particle reconstruction: theory, application and available software

•MicroED: theory, application and available software

•Cryo-electron tomography

•EPU single particle data collection tutorial and demonstration

•Model building, refinement, and validation.

•Cryo-FIB sample preparation tutorial and demonstration

•SerialEM electron tomography data collection tutorial and demonstration

•Single-particle data analysis workflow tutorial and demonstration

•Tomographic data analysis workflow including subtomography average tutorial and demonstration.